KMID : 0980320220220040277
|
|
Journal of Dental Anesthesia and Pain Medicine 2022 Volume.22 No. 4 p.277 ~ p.287
|
|
Dexmedetomidine and LPS co-treatment attenuates inflammatory response on WISH cells via inhibition of p38/NF-¥êB signaling pathway
|
|
Kim Tae-Sung
Yoon Ji-Young Kim Cheul-Hong Choi Eun-Ji Kim Yeon-Ha Kim Eun-Jung
|
|
Abstract
|
|
|
Background: Inflammatory dental diseases that occur during pregnancy can cause preterm labor and/or intrauterine growth restriction. Therefore, proactive treatment of dental diseases is necessary during pregnancy. Dexmedetomidine (DEX) is a widely used sedative in the dental field, but research on the effect of DEX on pregnancy is currently insufficient. In this study, we investigated the effects of co-treatment with DEX and lipopolysaccharide (LPS) on inflammatory responses in human amnion-derived WISH cells.
Methods: Human amnion-derived WISH cells were treated with 0.001, 0.01, 0.1, and 1 ¥ìg/mL DEX with 1 ¥ìg/mL LPS for 24 h. Cytotoxicity of WISH cells was evaluated by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. The protein expression of cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), p38, and nuclear factor kappa B (NF-¥êB) was examined by western blot analysis. The mRNA expression of pro-inflammatory cytokines such as interleukin (IL)-1¥â and tumor necrosis factor (TNF)-¥á was analyzed by real-time quantitative polymerase chain reaction.
Results: Co-treatment with DEX and LPS showed no cytotoxicity in the WISH cells. The mRNA expression of IL-1¥â and TNF-¥á decreased after co-treatment with DEX and LPS. DEX and LPS co-treatment decreased the protein expression of COX-2, PGE2, phospho-p38, and phospho-NF-¥êB in WISH cells.
Conclusion: Co-treatment with DEX and LPS suppressed the expression of COX-2 and PGE2, as well as pro-inflammatory cytokines such as IL-1¥â and TNF-¥á in WISH cells. In addition, the anti-inflammatory effect of DEX and LPS co-treatment was mediated by the inhibition of p38/NF-¥êB activation.
|
|
KEYWORD
|
|
Cyclooxygenase 2 Inhibitors, Dexmedetomidine, Lipopolysaccharides, Prostaglandin E2, WISH Cells
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|